Due to the need of high-speed and efficient biodosimetric assays for triage and therapy in the event of radiological or nuclear attack, a robotically based automated biodosimetry tool (RABiT) has been developed over the past few years. Adapting the micronucleus assay from filter plates to V-shaped plates presented challenges in the liquid handling, namely, cell splashing out of the V-shaped well plate during the cell harvesting, poor cell distribution on the bottom of the image plate during the dispensing, and cell loss from the image plate during the aspiration in the liquid handling process. Experimental and numerical investigations were carried out to better understand the phenomena and mitigate the problems. Surface tension and contact angle among the fluids and the plate wall were accounted for in the discrete and multiphase numerical models. Experimental conditions were optimized based on the numerical results showing the relationship between nozzle speed and amount of splashed liquid, and the relationship between aspiration speed and number of escaped cells. Using these optimized parameters, numbers of micronuclei in binucleated cells showed the same dose dependence in the RABiT-prepared samples as those in the manually prepared ones. Micronucleus assay protocol was fully realized on RABiT.
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December 2016
Research-Article
Liquid Handling Optimization in High-Throughput Biodosimetry Tool
Dakai Bian,
Dakai Bian
Department of Mechanical Engineering,
Columbia University,
New York, NY 10027
e-mail: db2875@columbia.edu
Columbia University,
New York, NY 10027
e-mail: db2875@columbia.edu
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Jason C. Tsui,
Jason C. Tsui
Department of Mechanical Engineering,
Columbia University,
New York, NY 10027
Columbia University,
New York, NY 10027
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Mikhail Repin,
Mikhail Repin
Center for Radiological Research,
Columbia University Medical Center,
New York, NY 10032
Columbia University Medical Center,
New York, NY 10032
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Guy Garty,
Guy Garty
Center for Radiological Research,
Columbia University Medical Center,
New York, NY 10032
Columbia University Medical Center,
New York, NY 10032
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Helen Turner,
Helen Turner
Center for Radiological Research,
Columbia University Medical Center,
New York, NY 10032
Columbia University Medical Center,
New York, NY 10032
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Y. Lawrence Yao,
Y. Lawrence Yao
Department of Mechanical Engineering,
Columbia University,
New York, NY 10027
Columbia University,
New York, NY 10027
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David J. Brenner
David J. Brenner
Center for Radiological Research,
Columbia University Medical Center,
New York, NY 10032
Columbia University Medical Center,
New York, NY 10032
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Dakai Bian
Department of Mechanical Engineering,
Columbia University,
New York, NY 10027
e-mail: db2875@columbia.edu
Columbia University,
New York, NY 10027
e-mail: db2875@columbia.edu
Jason C. Tsui
Department of Mechanical Engineering,
Columbia University,
New York, NY 10027
Columbia University,
New York, NY 10027
Mikhail Repin
Center for Radiological Research,
Columbia University Medical Center,
New York, NY 10032
Columbia University Medical Center,
New York, NY 10032
Guy Garty
Center for Radiological Research,
Columbia University Medical Center,
New York, NY 10032
Columbia University Medical Center,
New York, NY 10032
Helen Turner
Center for Radiological Research,
Columbia University Medical Center,
New York, NY 10032
Columbia University Medical Center,
New York, NY 10032
Y. Lawrence Yao
Department of Mechanical Engineering,
Columbia University,
New York, NY 10027
Columbia University,
New York, NY 10027
David J. Brenner
Center for Radiological Research,
Columbia University Medical Center,
New York, NY 10032
Columbia University Medical Center,
New York, NY 10032
1Corresponding author.
Manuscript received September 1, 2015; final manuscript received April 13, 2016; published online August 31, 2016. Assoc. Editor: Rafael V. Davalos.
J. Med. Devices. Dec 2016, 10(4): 041007 (10 pages)
Published Online: August 31, 2016
Article history
Received:
September 1, 2015
Revised:
April 13, 2016
Citation
Bian, D., Tsui, J. C., Repin, M., Garty, G., Turner, H., Lawrence Yao, Y., and Brenner, D. J. (August 31, 2016). "Liquid Handling Optimization in High-Throughput Biodosimetry Tool." ASME. J. Med. Devices. December 2016; 10(4): 041007. https://doi.org/10.1115/1.4033600
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