A parametric study was performed to understand the effect of preparation parameters on size, morphology, and encapsulation efficiency (i.e., cells/microcapsule) of alginate microcapsules prepared using the electrostatic spray method. The preparation parameters studied include sodium alginate concentration, spray voltage, flow rate, and cell density. It was found that both the flow rate and spray voltage have a significant impact on microcapsule size while the microcapsule morphology is greatly influenced by both the sodium alginate concentration and spray voltage. To obtain small cell-loaded microcapsules with good morphology (i.e., round in shape and uniform in size) and high encapsulation efficiency ( cells/microcapsule), the optimal ranges of spray voltage, flow rate, alginate concentration, and cell density are from 1.6–1.8 kV, 1.5–3 ml/h, >1.5% (w/v), and (3–5)106 cells/ml, respectively. Under optimal preparation conditions, cells were found to survive the microencapsulation process well.
Skip Nav Destination
e-mail: xmhe@sc.edu
Article navigation
July 2009
Technical Briefs
Encapsulation of Living Cells in Small Alginate Microcapsules by Electrostatic Spraying: A Parametric Study
Wujie Zhang,
Wujie Zhang
Department of Mechanical Engineering and Biomedical Engineering Program,
University of South Carolina
, 300 Main Street, Columbia, SC 29208
Search for other works by this author on:
Xiaoming He
Xiaoming He
Department of Mechanical Engineering and Biomedical Engineering Program,
e-mail: xmhe@sc.edu
University of South Carolina
, 300 Main Street, Columbia, SC 29208
Search for other works by this author on:
Wujie Zhang
Department of Mechanical Engineering and Biomedical Engineering Program,
University of South Carolina
, 300 Main Street, Columbia, SC 29208
Xiaoming He
Department of Mechanical Engineering and Biomedical Engineering Program,
University of South Carolina
, 300 Main Street, Columbia, SC 29208e-mail: xmhe@sc.edu
J Biomech Eng. Jul 2009, 131(7): 074515 (6 pages)
Published Online: June 30, 2009
Article history
Received:
September 12, 2008
Revised:
May 7, 2009
Published:
June 30, 2009
Citation
Zhang, W., and He, X. (June 30, 2009). "Encapsulation of Living Cells in Small Alginate Microcapsules by Electrostatic Spraying: A Parametric Study." ASME. J Biomech Eng. July 2009; 131(7): 074515. https://doi.org/10.1115/1.3153326
Download citation file:
Get Email Alerts
Related Articles
Thermal Spray Coating on Polymeric Composite for De-Icing and Anti-Icing Applications
J. Manuf. Sci. Eng (October,2021)
Effect of Elevated Temperature and Pressure on Sprays From Simplex Swirl Atomizers
J. Eng. Gas Turbines Power (January,1986)
Spray Characterization and Turbulence Properties in an Isothermal Spray With Swirl
J. Eng. Gas Turbines Power (January,1990)
Plasma-Spray Coating Processes: Physico-Mathematical Characterization
J. Eng. Power (July,1980)
Related Proceedings Papers
Related Chapters
Machine Vision Based Spraying Control of Agricultural Machinery
International Conference on Computer Technology and Development, 3rd (ICCTD 2011)
Antilock-Braking System Using Fuzzy Logic
International Conference on Mechanical and Electrical Technology, 3rd, (ICMET-China 2011), Volumes 1–3
A Calibrated Method for Crystallinity Determination of Hydroxylapatite Coatings
Characterization and Performance of Calcium Phosphate Coatings for Implants