Successful protocols for cryopreservation of living cells can be designed if the physicochemical conditions to preclude intracellular ice formation (IIF) can be defined. Unfortunately, all attempts to predict the probability of IIF have met with very limited success. In this study, an analytical model is developed to predict ice formation inside mouse oocytes subjected to a freezing stress. According to the model, IIF is catalyzed heterogeneously by the plasma membrane (i.e., surface catalyzed nucleation, SCN). A local site on the plasma membrane is assumed to become an ice nucleator in the presence of the extracellular ice via its effects on the membrane. This interaction is characterized by the contact angle between the plasma membrane and the ice cluster. In addition, IIF is assumed to be catalyzed at temperatures below -30° C by intracellular particles distributed throughout the cell volume (i.e., volume catalyzed nucleation, VCN). In the present study, these two distinctly coupled modes of IIF, especially SCN, are applied to various experimental protocols from mouse oocytes. Excellent agreement between predictions and observations suggests that the proposed model of IIF is adequate.
Skip Nav Destination
Article navigation
Research Papers
Cellular Response of Mouse Oocytes to Freezing Stress: Prediction of Intracellular Ice Formation
M. Toner,
M. Toner
Surgical Research Laboratory, Massachusetts General Hospital, Department of Surgery, Harvard Medical School, Charlestown, MA 02129
Search for other works by this author on:
E. G. Cravalho,
E. G. Cravalho
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139
Search for other works by this author on:
M. Karel
M. Karel
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139
Search for other works by this author on:
M. Toner
Surgical Research Laboratory, Massachusetts General Hospital, Department of Surgery, Harvard Medical School, Charlestown, MA 02129
E. G. Cravalho
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139
M. Karel
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139
J Biomech Eng. May 1993, 115(2): 169-174 (6 pages)
Published Online: May 1, 1993
Article history
Received:
October 1, 1991
Revised:
June 15, 1992
Online:
March 17, 2008
Connected Content
A companion article has been published:
Modelling Complex Force Systems, Part 2: A Decomposition of the Pad Forces in Deep Drilling
Citation
Toner, M., Cravalho, E. G., and Karel, M. (May 1, 1993). "Cellular Response of Mouse Oocytes to Freezing Stress: Prediction of Intracellular Ice Formation." ASME. J Biomech Eng. May 1993; 115(2): 169–174. https://doi.org/10.1115/1.2894117
Download citation file:
Get Email Alerts
Estimation of Joint Kinetics During Manual Material Handling Using Inertial Motion Capture: A Follow-Up Study
J Biomech Eng (February 2025)
Effect of Compressive Strain Rates on Viscoelasticity and Water Content in Intact Porcine Stomach Wall Tissues
J Biomech Eng (February 2025)
Eyelid Motion Tracking During Blinking Using High-Speed Imaging and Digital Image Correlation
J Biomech Eng (January 2025)
Related Articles
Network Thermodynamic Modeling With Bond Graphs for Membrane Transport During Cell Freezing Procedures
J. Heat Transfer (November,1988)
Survival of Biological Cells Deformed in a Narrow Gap
J Biomech Eng (December,2002)
Thermodynamic Nonequilibrium Phase Change Behavior and Thermal Properties of Biological Solutions for Cryobiology Applications
J Biomech Eng (April,2004)
The Thermodynamics of Water Transport From Biological Cells During Freezing
J. Heat Transfer (November,1975)
Related Proceedings Papers
Related Chapters
Chitosan-Based Drug Delivery Systems
Chitosan and Its Derivatives as Promising Drug Delivery Carriers
Further Applications of Spreading Resistance
Thermal Spreading and Contact Resistance: Fundamentals and Applications
Thermal Creep of Irradiated Zircaloy Cladding
Zirconium in the Nuclear Industry: Fourteenth International Symposium